Figure 8
From: Genomic identification and expression analysis of nuclear pore proteins in Malus domestica
Interaction between MdNup62 and MdNup54. (A) MdNup62508–613 interacted with MdNup54 in Y2H assays. The MdNup62508–613 truncated sequence was cloned into pGBKT7, whereas MdNup54 was cloned into pGADT7. Empty pGADT7 plus MdNup62508–613-pGBKT7 was used as a control. Yeast cells grown in SD/ − Trp/ − Leu medium and SD/ − Trp/ − Leu/ − His/ − Ade/ + X-α-gal medium are presented. (B) The luciferase complementation experiment involving tobacco leaves revealed the interaction between MdNup62 and MdNup54. Empty NLUC and empty CLUC, MdNup62-NLUC and empty CLUC, and empty NLUC + MdNup54-CLUC were used as controls. The luciferase complementation experiment was repeated three times, with consistent results. Asterisks denote significant differences as determined by the t-test: *P < 0.01.
