Figure 7

Disturbed Ca²⁺ homeostasis and ROS production regulated by zebrafish HIF-1aa and HIF-1ab. (a) Cells were stained with Fluo-4 AM to visualize intracellular Ca²⁺. Fluorescence intensity is correlated with the intracellular Ca²⁺ concentration. (b) Statistical analysis shows that HIF-1aa induced a decrease in the intracellular Ca²⁺ concentration. Data are given as mean ± SE (n = 4 per group). *p < 0.05. (c) ROS generation regulated by HIF-1aa. Cells were stained with 10 μM DCHF diacetate for 30 min. Oxidative metabolism was reflected in the conversion to dichlorofluorescein (DCF). Fluorescence was determined using a multifunctional microplate reader. Data are given as mean ± SE (n = 3 per group). **p < 0.01.