Figure 6
Direct quantification of total glycogen and lipid in Cx. pipiens by FTIR. (a) ATR-FTIR spectra of dsi-control, dsi-glys, dsi-ldlr, and dsi-atp-treated diapausing females of Cx. pipiens fed with 10% Glc for 7-days post adult eclosion. (b) The FTIR spectra were normalized to the amide I intensity at 1631 cm−1. (c) The absorption of lipid 2800–3000 cm−1 shows a large reduction of lipid accumulations in mosquitoes that were treated with dsi-RNAs that targeted the downstream elements of Foxo. (d) Polysaccharide regions show reduced glycogen accumulations in dsi-RNA treated mosquitoes.
