Figure 2
TEC-1 does not substantially affect FOXM1 splicing. (a) Schematic representation of exon construction and exon inclusion of FOXM1 mRNA splicing. (b) RT-PCR analysis of FOXM1 variants of GM03813 fibroblasts exposed to each compound for 24 h. RT-PCR products of FOXM1 variants were separated by agarose gel electrophoresis and visualized with ethidium bromide. Standard FOXM1 fragments corresponding to three isoforms (Oligo STD) are shown. Molecular weight markers are shown on the right (bp). The full-length gel is presented in Supplementary Fig. 1e. (c–e) qPCR analysis of each FOXM1 variant of GM03813 cells exposed to each compound for 24 h. The amounts of each variant were normalized to those of GAPDH. Data are from two biologically independent samples in (c–e).
