Figure 2

The role of SOD1 and SOD3 antioxidant enzymes in the functions of AT-MSCs. (A) The protein expression of antioxidant enzymes in infant and elderly AT-MSCs. Infant AT-MSCs and elderly AT-MSCs were derived from 5 different donors, respectively (n = 5) and the comparison was conducted with infant AT-MSCs and elderly AT-MSCs at the same passage number. (B) The protein expression of wild-type and SOD1 knockdown infant AT-MSCs. The knockdown experiments were conducted with infant AT-MSCs from 3 different donors (n = 3). (C) The expression of ROS in wild-type and SOD1 knockdown infant AT-MSCs (n = 3). (D) The cellular senescence of wild-type and SOD1 knockdown infant AT-MSCs (n = 3). (E) Transplantation of wild-type and SOD1 knockdown infant AT-MSCs to an in vivo streptozotocin-induced diabetic ischemic flap mouse model (n = 3). (F) The protein expression of wild-type and SOD3 knockdown infant AT-MSCs. (G) The expression of ROS in wild-type and SOD3 knockdown infant AT-MSCs (n = 3). (H) The cellular senescence of wild-type and SOD3 knockdown infant AT-MSCs (n = 3). (I) Transplantation of wild-type and SOD3 knockdown infant AT-MSCs to an in vivo streptozotocin-induced diabetic ischemic flap mouse model (n = 3). (J) The protein expression of wild-type elderly AT-MSCs or with the overexpression of SOD1 or SOD3. The overexpression experiments were conducted with elderly AT-MSCs from 3 different donors (n = 3). (K) The ROS expression in wild-type elderly AT-MSCs or with the overexpression of SOD1 or SOD3 (n = 3). (L) The cellular senescence of wild-type elderly AT-MSCs or with the overexpression of SOD1 or SOD3 (n = 3). (M) The mRNA expression of wound healing-related growth factors in wild-type elderly AT-MSCs or with the overexpression of SOD1 or SOD3 (n = 3). (N) Transplantation of wild-type elderly AT-MSCs or with the overexpression of SOD1 or SOD3 to a streptozotocin-induced in vivo diabetic ischemic flap mouse model (n = 3). WT: wild-type, SOD1 Kd: knockdown of SOD1, SOD3 Kd: knockdown of SOD3, SOD1: overexpression of SOD1, SOD3: overexpression of SOD3. The data represent the mean ± SD. ***P <0.001, **P < 0.01, *P < 0.05, ns no significance. The experiments were performed in triplicate. Full-length Western blots are shown in Supplementary Figure S3.