Figure 6

Low-grade inflammation. Duodenal biopsy samples after placebo (black dots) and DSCG treatment (white dots), were stained for eosinophils using eosinophilic MBP (A,B) and for mast cells using tryptase (C,D). Representative images of MBP (B) and tryptase (D) immunohistochemistry in mucosal biopsy specimens obtained after placebo (left) and DSCG treatment (right). Scale bar: 20 µm. (E) Protein expression of MBP and tryptase was measured by western blot after intraduodenal acid perfusion with placebo (black dots) and DSCG (white dots) pretreatment. (n = 10 for both groups). (F) Representative western blot of five saline perfused and five acid perfused subjects. Bands were cropped from different parts of the same gel, or from different gels. Data are mean ± SEM. MBP, eosinophilic major basic protein.