Table 1 mRNA expression of cell-to-cell adhesion proteins and eosinophil and mast cell markers.

From: Duodenal acidification induces gastric relaxation and alters epithelial barrier function by a mast cell independent mechanism

Gene

Saline

Acid

P value

CLDN1

1.06 ± 0.14

0.93 ± 0.13

0.39

CLDN2

0.82 (0.65–2.08)

0.93 (0.57–1.60)

0.74

CLDN3

1.08 (0.87–1.19)

1.00 (0.76–1.02)

1.00

CLDN4

1.02 ± 0.07

1.15 ± 0.13

0.27

OCLN

1.03 ± 0.09

0.91 ± 0.09

0.29

ZO1

1.01 ± 0.05

1.07 ± 0.13

0.81

ZO2

1.02 ± 0.07

0.97 ± 0.10

0.84

ZO3

1.02 ± 0.06

0.96 ± 0.12

0.83

β-catenin

1.00 ± 0.03

0.93 ± 0.07

0.53

E-cadherin

1.02 ± 0.06

0.89 ± 0.12

0.46

DSC2

1.02 ± 0.06

0.95 ± 0.10

0.80

DSG2

1.05 ± 0.11

0.85 ± 0.15

0.18

PRG2

1.04 ± 0.11

0.93 ± 0.08

0.81

TPSAB1

2.08 ± 0.58

1.60 ± 0.56

0.20

  1. Real-time RT-PCR was used to evaluate the gene expression of cell-to-cell adhesion proteins (upper panel) and an eosinophil marker (PRG2) and a mast cell marker (TPSAB1) (lower panel). Data are mean ± SEM or median (IQR). CLDN, claudin; OCLN, occludin; ZO, zonula occludens; DSC2, desmocollin-2; DSG2, desmoglein-2; PRG2, eosinophil major basic protein; TPSAB1, tryptase Alpha/Beta 1.
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