Figure 1 | Scientific Reports

Figure 1

From: Pharmacological characterisation of novel adenosine A3 receptor antagonists

Figure 1The alternative text for this image may have been generated using AI.

Characterisation of A3R antagonist at all AR subtypes. A3R Flp-In CHO cells or CHO-K1 cells (2000 cells/well) stably expressing one of the remaining AR subtypes were exposed to forskolin in the case of Gi-coupled A1R and A3R (1 μM or 10 μM, respectively) or DMSO control in the case of Gs-coupled A2AR and A2BR, NECA and test compound (10 μM) for 30 min and cAMP accumulation detected. All values are mean ± SEM expressed as percentage forskolin inhibition (A1R and A3R) or stimulation (A2AR and A2BR), relative to NECA. n ≥ 3 independent experimental repeats, conducted in duplicate.

Back to article page