Figure 4
RSV is protective against CLP model of polymicrobial sepsis. (A) Bacteria counts in whole blood and peritoneal lavage fluid of sham-operated mice, CLP mice injected with PBS (CLP + PBS) and CLP mice and injected with 1 mg/kg of RSV (CLP + RSV) were assessed by colony-forming unit method 24 h after CLP (n = 5 for each condition). Statistical analysis was performed using one-way ANOVA followed by post hoc Tukey’s multiple comparisons test. .PBS-treated mice were compared to RSV-treated mice (**** p < 0.0001). (B) IL-3 and TNFα in serum of mice treated as in A, were measured 24 h after CLP by ELISA (***p < 0.001, ****p < 0.0001). Statistical analysis was performed using one-way ANOVA followed by post hoc Tukey’s multiple comparisons test. (C) Serum HMGB1 levels of mice treated as in A were measure by ELISA 24 h after CLP (n = 3 for each condition; *p < 0.05; **p < 0.01). Statistical analysis was performed using one-way ANOVA followed by post hoc Tukey’s multiple comparisons test. (D) Numbers of neutrophils infiltrated into peritoneum of mice treated as in A were counted at 2 h and 6 h after CLP (n = 3 for each condition). Statistical analysis was performed using two-way ANOVA followed by post hoc Tukey’s multiple comparisons test RSV-treated group was compared to PBS-treated group (*p < 0.05). Data were derived from Dr. Wang Binbin PhD Thesis36.
