Figure 5
RSV inhibits pro-inflammatory cytokines production by human monocytes from post-TRUS biopsy patients. (A) Monocytes isolated from blood of patients before (Pre) and 24 h post-TRUS (Post) biopsies were cultured. Supernatants were harvested 24 h later to quantify for IL-8, TNF-α, IL-1β and IL-6 levels by ELISA. Each data point represents 1 patient sample and respective p-values shown are post-biopsy compared to pre-biopsy. Statistical analysis was performed using two-tailed paired t test. (B) Monocytes isolated from post-TRUS biopsy blood samples were either left untreated (Ctrl) or treated with 20 µM or 40 µM RSV. Supernatants were harvested 24 h later to quantify for IL-8, TNF-α, IL-1β and IL-6 levels by ELISA. Data shown are mean ± SD of n ≥ 4 and * depicts p < 0.05 compared to respective control samples. Statistical analysis was performed using one-way ANOVA followed by post hoc Tukey’s multiple comparisons test. (C) Monocytes from healthy donors either untreated or pre-treated with 40 µM RSV were stimulated with LPS for 24 h before measurement of IL-8, TNF-α, IL-1β and IL-6 levels by ELISA. Each data point represents 1 patient sample. Data shown is fold change with respect to samples not treated with LPS and respective p-values shown are samples pre-treated with RSV compared to non-treated samples. Statistical analysis was performed using two-tailed paired t test.
