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Figure 1

From: Neonatal monocytes demonstrate impaired homeostatic extravasation into a microphysiological human vascular model

Figure 1

3D vascular-interstitial model. (a) Type I collagen is cast into cushions in a 96-well microtiter plate; (b) Single-donor human primary endothelial cells are grown over the cushions until achieving confluency (representative image); (c) CD33-purified human monocytes (representative histograms for CD14 expression) are then added to confluent tissue constructs (TCs) to enable autonomous extravasation for 1.5 h at 37 °C/5%CO2; (d) non-migrated cells are then removed by gentle aspirations; (e) TCs are then examined by microscopy or digested to extract extravasated monocytes for further analysis. I Isotype control. HPF High-Power Field. Scale bars = ∼200 μm.

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