Figure 2
Newborn monocytes demonstrate lower adherence and extravasation as compared to adult monocytes. Purified human monocytes from either a newborn or an adult study participant were applied to each confluent TC well to enable autonomous extravasation under corresponding 100% autologous untreated plasma for 1.5 h at 37 °C/5%CO2, as described in methods. (a) Newborn and adult monocytes adhered to the endothelium (bright dots indicated by arrows) and extravasated (dark dots indicated by arrows) after removal of non-migrated cells from the top of TCs (representative images taken at surface level and at ~ 80 mm inside the constructs). Scale bar = ∼200 μm. (b) Index of adherence to quiescent endothelium, obtained as described in “Methods” (N = 3/age-group). (c) Number of viable extravasated monocytes from digested TCs (N = 6/age-group). Unpaired t-Test, Two-tails * = P ≤ 0.05; *** = P ≤ 0.005.
