Figure 4
Cytokines and chemokines released by endothelium in the presence of autologous plasmas. Cytokines and chemokines were measured on study participant’s autologous plasmas (N = 3/age-group) after being over either quiescent or stimulated TCs devoid of monocytes for 12 h at 37 °C/5%CO2. 1 mg/mL LPS was used to stimulate TCs for 12 h, as described in methods. Unstimulated controls (Ø) received 1% DMSO. (a) Microscopic analysis of endothelium after stimulation (representative images, 4X). (b) Analytes measured were IL-1β, IL-6, CXCL8 (IL-8), IL-10, IL-12p40, IL-12p70, TNF-α, IFN-α2, IFN-γ, CXCL-10 (IP-10), CCL-2 (MCP-1), GM-CSF, CCL-3 (MIP-1α) and CCL-5 (RANTES). Chemokines CCL-2 and CXCL-8 in stimulated group reached their maximum detection limit. Cytokines IL-1β, IL-10, IL-12p40, IL-12p70, TNF-α, IFN-α2 and IFN-γ were not detected in both age groups. UnPaired t-Test, two-tailed *P ≤ 0.05; **P ≤ 0.01.
