Figure 1
Prenatal mammary gland development in mice with embryonal Sox10 deletion. (a–g) X-gal staining was performed at E14.5 (a,b), E16.5 (c,d) and E18.5 (e–g) on ventral dermis including mammary gland anlagen to detect β-galactosidase activity in Sox10+/lacZ (a,c,f), Sox10lacZ/lacZ (b,d,g) and control Sox10::Cre Rosa26stopflox-lacZ (e) mouse embryos. Staining is in the mammary gland anlagen (asterisks) and peripheral nerves (arrowheads). Magnifications in right upper corner (a–d) or on right (e–g) correspond to boxed areas in main panels. (h–v) Confocal sections of mammary gland ducts at E18.5 (h–q) and P56 (r–v) in Sox10lacZ/lacZ (h–l) and Sox10+/+ (m–v) mice. Immunohistochemistry was performed using antibodies directed against keratin 14 (h,m,r), keratin 8 (i,n,s), β-galactosidase (βGal, j) and Sox10 (o,t). Single (h–j,m–o,r–t) or merged (k,l,p,q,u,v) stainings are shown. Scale bars: 50 µm (b,d), 2 mm (g), 10 µm (l,q,v).
