Figure 3
Mammary gland development in mice with early postnatal Sox10 deletion. (a) Mammary glands (schematically shown in the upper left panel including lymph node, LN) of MMTV::Cre(A) Rosa26stopfloxYFP mice had undergone efficient Cre-dependent recombination at P21 as evident from YFP signals in whole mounts (upper right panel, corresponding to boxed area) and epithelial duct cross-sections (left lower panel) as well as quantification of YFP-positive cells ± SEM in the mammary epithelium (n = 3). DAPI was used to counterstain nuclei. (b) Ductal morphology in female control and MMTV::Cre(A) Sox10fl/fl mice was analyzed by hematoxylin/eosin staining at P56. (c–h) Carmine-alum staining was used to visualize the mammary epithelium in female MMTV::Cre(A) Sox10fl/fl mice at P42 (c,d) and P56 (e,f) or after 1 day of lactation (g,h). Panels on the left show the whole gland; panels on the right are magnifications of the area containing the mammary gland epithelium. Scale bars: 1 mm (a, upper panel), 100 µm (a, lower panel; b), 2 mm (c), 200 µm (d).
