Figure 3
Loss of PI-PLC does not affect Staphylococcus aureus-induced skin inflammation in normal mice. (a) Epidermal thickness at 4 days after epicutaneous infection of S. aureus. N = 5 in each group. The data corresponding to the right and left flank of the same mice were linked with lines. Data are presented as the means ± SEM. Individual data values are represented by a single symbol on the bar graphs. (b) Staining of skin specimens with hematoxylin and eosin (top panels) or with antibodies against CD45 (red), Gr-1 (red), CD4 (red), and Hoechst (blue) at 4 days after epicutaneous infection by S. aureus. Staining was also performed on non-infected skin (non). Scale bar = 50 µm. Images are representative of five animals per group. Images of wild-type- and Δplc mutant-infected skin in (b) are from the right and left flanks of the same mice, respectively. Results are representative of two technical replicates.
