Figure 2

mRNA and protein expression of CysLTR1 in polarized ARPE-19 cells. CysLTR1 expression upon treatment with 300 µM H₂O₂ for 3 h in polarized (7–9 days) ARPE-19 cells at the (a) mRNA and (b) protein levels detected by qPCR and western blot analysis, respectively. (c) Representative western blot analysis showing total protein loading and CysLTR1 expression in untreated polarized ARPE-19 cells and polarized ARPE-19 cells treated with H₂O₂ for 3 h. The values are represented in box and whisker plot format (min to max); n = 15 (mRNA), n = 12 (protein). Significance was calculated by paired t-test. ***p < 0.001. Cq quantification cycle, GOI gene of interest, RG reference gene.