Figure 2 | Scientific Reports

Figure 2

From: Abrogation of HLA surface expression using CRISPR/Cas9 genome editing: a step toward universal T cell therapy

Figure 2

Efficient HLA disruption in human T cells by multiplex genome editing. Primary human CD3+ T cells were transfected with a mixture of gRNAs targeting B2M, HLA-DRA, HLA-DQA and HLA-DPA (Quadruple gRNAs) or non-targeting gRNA (control gRNA) together with Cas9 protein and expanded for 13 days ex vivo. (A) Representative histograms of HLA expression on CD4+ (left) or CD8+ (right) T cells gated from 13-day expanded CD3+ T cells. (B) Quantification of HLA expression in CD4+ (top) and CD8+ (bottom) T cells in (A) (pooled results from three independently performed experiments, total n = 6). Percentages of target HLA-positive cells out of total cells are presented as % HLA pos. Error bars indicate mean ± SEM. *, p = 0.0313, by Wilcoxon matched-pairs signed rank test.

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