Figure 2 | Scientific Reports

Figure 2

From: Response of human melanoma cell lines to interferon-beta gene transfer mediated by a modified adenoviral vector

Figure 2

Characterization of cell death in response to adenoviral hIFNβ gene transfer. SK-MEL-05 and SK-MEL-147 cells were transduced with AdRGD-CMV-LacZ (LacZ) or AdRGD-PG-hIFNβ (hIFNβ) using an MOI of 100 or non-treated cells (Mock) were used as a control. In the upper panel, after 24, 48 or 72 h incubation, cells were harvested and stained with annexinV/PI. Graphs show percentage of the total cell population staining positive for annexinV + annexinV/PI. Alternatively, in the lower panel, the cells were tested for caspase 3/7 activity after 60 h incubation. In either case, graphs depict the mean and standard deviation from three independent assays each performed with technical duplicates. Statistical analyses were performed by the One-way ANOVA test followed by the Tukey’s range test using GraphPad Prism version 5.0 for Windows (GraphPad Software, La Jolla California USA). * p < 0.01.

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