Figure 9

DE-71 exposure increases hepatic levels of endocannabinoid (EC) and related fatty acid-ethanolamides in exposed F1 but not F0 female mice. Post mortem liver tissue was analyzed using UPLC/MS/MS. Perinatal (a, b) but not adult exposure to DE-71 (c,d) produced elevated levels of hepatic ECs and fatty acid-ethanolamides. F1 exposed to 0.1 mg/kg displayed elevated DHEA and OEA (a). F1 exposed to 0.4 mg/kg showed elevated AEA (Anandamide), a primary EC, and DHEA. No significant differences were detected after adult exposure to DE-71 in F0 (c,d). The other primary EC, 2-AG and 2-DG did not exhibit changes in DE-71 exposed F1 (b) or F0 mice (d). *Indicate significantly different from VEH/CON (*P < .05). Bars and error bars represent mean ± s.e.m. n = 6–16/group for F1 and n = 3–5/group for F0. Dunnett’s T3 and Tukey’s post-hoc test was used. AEA arachidonoylethanolamide (Anandamide), DHEA docosahexanoyl ethanolamide, OEA n-oleoyl ethanolamide, 2-AG 2-arachidonoyl-sn-glycerol, 2-DG monoacylglycerol 2-docosahexaenoyl-sn-glycerol, F1 female offspring, F0 dams.