Figure 3 | Scientific Reports

Figure 3

From: Rapid production of SARS-CoV-2 receptor binding domain (RBD) and spike specific monoclonal antibody CR3022 in Nicotiana benthamiana

Figure 3

SDS-PAGE and western blot analysis of RBD protein of SARS-CoV-2 produced in N. benthamiana. The crude proteins were extracted from plants, and the RBD protein was purified, analyzed on SDS-PAGE gels and visualized with InstantBlue™ (a). Lane M: protein ladder; Lane 1: total soluble protein of N. benthamiana agroinfiltrated with pBY2e-SARS-CoV-2 RBD; Lane 2: purified SARS-CoV-2 RBD. For western blot analysis, proteins on the blot were probed with a rabbit anti-his antibody conjugated with HRP (b). Lane 1: crude extract from non-infiltrated N. benthamiana; Lane 2: total soluble protein of N. benthamiana agroinfiltrated with pBY2e-SARS-CoV-2 RBD; Lane 3: purified SARS-CoV-2-RBD.

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