Figure 3
From: TGF-β1 is a regulator of the pyruvate dehydrogenase complex in fibroblasts
Effect of TGF-β1 on acetyl-CoA and fibroblast activation. (A) Enzymatic measurement of free acetyl-CoA concentrations (B), α-SMA and (C), pro-collagen I levels in UUOF treated for 24 h with TGF-β1 (1 ng/mL) or CPI-613 (100 µM) compared to control (pooled data from 3 independent experiments). Measurements in (A) were normalised for total protein content. Plots shows mean ± SEM. *P < 0.05; ***P < 0.001; ****P < 0.0001. P-values were determined using Brown-Forsyth and Welch one-way ANOVA with Dunnett’s T3 multiple comparisons test.
