Table 2 Analysis of TCN, nº of cells in the ICM and TE and AR of Day 8 blastocysts derived from oocytes IVM in a medium supplemented with 25 ng/mL of LIF.

From: In vitro maturation in the presence of Leukemia Inhibitory Factor modulates gene and miRNA expression in bovine oocytes and embryos

 

Day 8 blastocysts

n

TCN ± SEM

ICM cell number ± SEM

TE cell number ± SEM

AR ± SEM

Bl

Exp

Hd

Bl

Exp

Hd

Bl

Exp

Hd

Bl

Exp

Hd

Bl

Exp

Hd

Control (n = 47)

18

16

13

84.7 ± 2.6a,c

148.8 ± 5.3a,d

199.7 ± 9.1a,e

8.8 ± 0.8a,c

14.2 ± 0.7a,d

25.9 ± 2.5a,e

75.9 ± 2.1a,c

125.0 ± 3.8a,d

173.8 ± 8.6a,e

8.3 ± 0.3a,c

4.5 ± 0.2a,d

4.2 ± 0.4a,d

LIF (n = 62)

20

26

16

86.7 ± 3.1a,c

134.8 ± 4.4a,d

202.1 ± 6.1a,e

12.8 ± 1.0b,c

22.6 ± 1.8b,d

34.9 ± 2.3b,e

74.5 ± 2.4a,c

116.6 ± 4.7a,d

163.8 ± 6.7a,e

8.9 ± 0.7a,c

3.8 ± 0.1a,d

3.6 ± 0.5a,d

  1. Treatment groups: Control: bovine oocytes matured in TCM-199 supplemented with 10% FBS, 10 ng/mL EGF and 50 µg/mL gentamycin. LIF: bovine oocytes matured in TCM-199 supplemented with 10% FBS, 10 ng/mL EGF and 50 µg/mL gentamycin and 25 ng/mL of LIF.
  2. Bl blastocyst, Exp expanded blastocyst, Hd Hatching/Hatched blastocyst. Data are shown as mean ± SEM, TCN total cell number, ICM inner cell mass, TE trophectoderm, AR Apoptotic rate.
  3. a,bValues within columns with different superscripts differ significantly (P < 0.05); c,d,eValues for each valuable within rows with different superscripts differ significantly (P < 0.05).
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