Table 1 Nucleotide sequences of primers used in this study68,69.
From: Quercetin inhibits virulence properties of Porphyromas gingivalis in periodontal disease
Gene* | Description | Primer sequence (5′-3′) | |
|---|---|---|---|
Forward | Reverse | ||
16S rRNA | Normalizing internal standard | TGTAGATGACTGATGGTGAAA | ACTGTTAGCAACTACCGATGT |
ftn | Ferritin | CGGCGAGGTGAAGATAGAAG | CTCCTGAGAGAGACGGATCG |
hagA | Hemagglutinin protein HagA | TAAATAAGGGCGGAGCAAGA | GACGGAAAGCAACATACTTCG |
hagB | Hemagglutinin protein HagB | TGTCGCACGGCAAATATCGCTAAAC | CTGGCTGTCCTCGTCGAAAGCATAC |
hem | Hemolysin | ACGAAGCCTTGTTCTCCTCA | CAATGAATATGCCGGTTTCC |
hmuR | Heme/hemoglobin utilization receptor HmuR | CTCCCATGCGGCCAACCCTCC | GCAGACGGGCTGTACGGCTACC |
kgp | Lysine-specifc cysteine proteinase Kgp | AGGAACGACAAACGCCTCTA | GTCACCAACCAAAGCCAAGA |
rgpA | Arginine-specifc cysteine proteinase RgpA | CACCGAAGTTCAAACCCCTA | GAGGGTGCAATCAGGACATT |
rgpB | Arginine-specifc cysteine proteinase RgpB | GCTCGGTCAGGCTCTTTGTA | GGGTAAGCAGATTGGCGATT |
ragA | Receptor antigen A | CGCTATTCTTCCTTTGCTTGCT | GATCGTGGTGTTTCCGACAA |
vimA | Virulence modulating gene A | TCGCGTAGTCTGAGAGTAACCTT | GGTATAAACGAAGACAGCACGAC |
