Figure 5
From: Experimental adaptation of dengue virus 1 to Aedes albopictus mosquitoes by in vivo selection
Growth curves of the two passages 10 of DENV-1 1806 strain in three cell lines, (a) Ae. albopictus C6/36 cells, (b) Ae. albopictus U4.4 cells, and (c) human foreskin fibroblasts HFF cells. Cells were infected with the parental strain and the two replicates of the 10th passages of DENV-1 1806 (P10_R1 and P10_R2) at a MOI of 0.1. Supernatants were collected at 4, 6, 8, 10, 24, 48 and 72 h post-inoculation. The number of infectious viral particles was determined by focus fluorescent assay on Ae. albopictus C6/36 cells. Three replicates were performed for each cell-virus pairing. Error bars show standard deviations.
