Table 1 Kinetic constants Kd, ka, and kd of the wild-type and mutant GPs.
Mutations | Kd (nM) | ka (M−1 s−1) | kd (s−1) |
|---|---|---|---|
Wild-type | 0.07 ± 0.01 | 6.46 × 105 | 4.53 × 10−5 |
G238A | 0.58 ± 0.23 | 2.88 × 105 | 16.6 × 10−5 |
S239A | 2.74 ± 0.94 | 0.30 × 105 | 8.29 × 10−5 |
C240A | No binding | No binding | No binding |
S241A | 0.06 ± 0.03 | 5.50 × 105 | 2.99 × 10−5 |
R242A | 0.15 ± 0.002 | 2.95 × 105 | 4.52 × 10−5 |
L305A | 0.22 ± 0.08 | 3.98 × 105 | 8.17 × 10−5 |
F458A | 0.67 ± 0.09 | 1.77 × 105 | 11.70 × 10−5 |
P488A | 0.14 ± 0.06 | 3.33 × 105 | 4.64 × 10−5 |
G489A | 1.37 ± 0.14 | 3.96 × 105 | 53.5 × 10−5 |
Q490A | 0.07 ± 0.005 | 5.72 × 105 | 3.92 × 10−5 |
W504A | 2.09 ± 0.05 | 4.16 × 105 | 87.2 × 10−5 |
E505A | < 0.001 | 2.00 × 105 | < 1.0 × 10−7 |
G506A | No binding | No binding | No binding |
V507A | 0.02 ± 0.002 | 3.63 × 105 | 0.70 × 10−5 |
T531A | < 0.001 | 3.08 × 105 | < 1.0 × 10−7 |
A532W | No binding | No binding | No binding |
E533A | < 0.001 | 1.62 × 105 | < 1.0 × 10−7 |
E554A | < 0.001 | 2.49 × 105 | < 1.0 × 10−7 |
D555A | 0.09 ± 0.002 | 3.32 × 105 | 2.85 × 10−5 |
N557A | No binding | No binding | No binding |
A558W | No binding | No binding | No binding |
Q559A | < 0.001 | 6.37 × 105 | < 1.0 × 10−7 |
E579A | < 0.001 | 6.00 × 105 | < 1.0 × 10−7 |
Y581A | < 0.001 | 1.46 × 105 | < 1.0 × 10−7 |
T583A | 0.06 ± 0.01 | 5.05 × 105 | 3.08 × 10−5 |
D585A | 0.05 ± 0.01 | 3.39 × 105 | 1.85 × 10−5 |
N586A | 0.30 ± 0.07 | 2.50 × 105 | 7.44 × 10−5 |
I588A | 0.36 ± 0.13 | 1.36 × 105 | 5.06 × 10−5 |
