Figure 7
No change in migration of BCG in presence of strong VPP or naïve CM. (a) No differences were observed when comparing the migration of BCG through co-cultures treated with CM from naïve and strong VPP PBMCs (One way ANOVA; p ≥ 0.05). Data presented as Mean ± SD, n = 3. (b) Dextran permeability studies highlighted that OpTmizer CTS serum free medium (SFM) had a detrimental effect on the integrity of the co-culture, as indicated by an increase in DB2000 quantified in the basolateral chamber. Comparing groups to SFM as negative control, only one point of significance was determined, between the naïve (1406) −/− and + / + groups (One way ANOVA followed by Tukey’s Multiple Comparisons test; *P ≤ 0.05) Data presented as Mean ± SD, n = 3. (c) BPAEC (i) and BATII (ii) were cultured in EGM-2 alongside OpTmizer CTS serum free medium (SFM) (iii and iv respectively) for 48 h (the period of exposure of co-cultures to conditioned medium) on LabTek II chamberslides. A decrease in cells was observed for both BPAEC and BATII cultures in SFM, based upon morphological light microscopy analysis. Images representative of n = 4, objective 10 x.
