Figure 6

The effects of EEDK on the DNA binding activity of AP-1 and NFAT. (a,b) AP-1 activity of (a) HEK293A and (b) HepG2 cells exposed to the control (0.5% (v/v) DMSO) and EEDK (50 μg/mL) for 24 h. Cells were transfected with 3xAP1pGL3 (40342, Addgene). (c,d) NFAT activity of (c) HEK293A and (d) HepG2 cells exposed to the control (0.5% (v/v) DMSO) and EEDK (50 μg/mL) for 24 h. Cells were transfected with pGL3-NFAT luciferase (17870, Addgene). The bar graphs present mean values of relative luminescence values, with error bars indicating the S.E.M (n = 3, **P < 0.01, Student’s t-test). Luminescence values were detected using the Glomax Multi + Microplate Multi Reader (9301-010; Promega, USA).