Figure 6
From: Low dose amiodarone reduces tumor growth and angiogenesis
Amiodarone inhibits angiogenesis in Matrigel plugs. Matrigel containing VEGF and bFGF (control) or Matrigel containing VEGF and bFGF mixed with Amiodarone 0.05 mg/plug was injected S.C. into C57BL/6 mice to determine the drug’s effect on angiogenesis. (A, B) Representative plugs removed from Amiodarone-treated and untreated mice after 8 days. Untreated mice presented bloody plugs surrounded by massive blood vessels, compared with Amiodarone-treated mice which had poor vasculature. (C) Representative histologic sections of untreated plug (left) and Amiodarone-treated plug (right) of mice post anti-CD31 antibody (DAB-brown) and Gill’s Hematoxylin for nuclei (blue) staining. Arrows point to large vessels with open lumens. Scale bar: 50 µm. (D) Quantification of infiltrating endothelial cells in Matrigel plugs employing the flow cytometry assay. FACS dot plots of endothelial cells (CD31 + /CD45, marked in red squares) from single-cell suspension originated from untreated (left) and treated (right) Matrigel plugs. Data are presented as percent of specific cell population out of the total cell population (p < 0.05). Infiltrating endothelial cells were reduced by 60% in the Amiodarone-treated mice group compared with the non-treated control group. n = 3. Objective lens 400x.
