Figure 1
From: Identification of DHX9 as a cell cycle regulated nucleolar recruitment factor for CIZ1
Identification of CIZ1 interaction partners by MALDI MS/MS. (A) Schematic of murine CIZ1, showing translated exons (numbered), and those that encode the DNA replication domain encoded by CIZ1 fragment RD (exons 2–9 and part of 10, shown in yellow and red box)5. The nuclear matrix anchor domain-containing fragment (exon 10–17; grey box) is also shown7. Reported CIZ1 spicing events16,17,37,69 are shaded in dark grey in exons 4, 6, 8, and 14. (B) Four constructs were prepared for interaction screen: GST-ECIZ1, GST-ECIZ1 RD and DNA replication inactive GST-ECIZ1 N391. GST was used as a negative control. (C) MALDI-MS/MS and mascot database searching was used to identify interaction partners for CIZ1 (Table S1–4). STRING analysis68 of ECIZ1 and ECIZ1-RD shared Interaction partners identify two clusters: RNA binding/helicases (left) and ribosomal cluster (right). Functional interaction data is shown in key. (D) Common CIZ1-DHX9 interaction partners were analysed by STRING analysis. The proteins identified here share RNA binding/helicase activities and ribosomal subunits as seen for CIZ1.
