Figure 4
From: Identification of DHX9 as a cell cycle regulated nucleolar recruitment factor for CIZ1
CIZ1 localisation to the nucleolus requires DHX9. (A) Percentage of cells showing nucleolar localisation of CIZ1-DHX9 as determined by confocal microscopy. For triplicate experiments showing mean ± SD, where > 50 nuclei were scored for each time point. (B) Confocal immunofluorescence microscopy showing total DNA is shown in blue; DHX9, green; CIZ1, Red and merged image, with yellow showing colocalization, ± Actinomycin D. (C) Relative quantification of DHX9 transcript levels for control and DHX9 siRNA treated cells 24 h post-transfection. (D) Western blot analysis of DHX9 depleted cells showing levels of DHX9, CIZ1, nucleophosmin and actin protein. (E) Confocal fluorescence microscopy of HeLa cells showing CIZ1/DHX9 after control siRNA or DHX9 siRNA transfection. Total DNA is shown in blue; DHX9, green; CIZ1, Red and merged image, with yellow showing colocalisation. (F) Nucleolar localisation of nucleophosmin (B23) is independent of DHX9 expression. Total DNA is shown in blue; DHX9, green; nucleophosmin (B23), Red and merged image, with yellow showing colocalisation. (G) Nucleolar localisation of UBF is independent of DHX9 expression. Total DNA is shown in blue; UBF, green; CIZ1, red and merged image, with yellow showing colocalisation. (H) Percentage of cells showing nucleolar localisation of CIZ1-DHX9 as determined by confocal microscopy data shows mean ± SD, from 3 independent experiments each with > 50 nuclei scored.
