Table 1 Top 30 genes expressed in cryptal mesenchymal cells.

From: Stratified layer analysis reveals intrinsic leptin stimulates cryptal mesenchymal cells for controlling mucosal inflammation

Rank

In vivo upper mucosa

In vivo cryptal layer

Ex vivo cryptal layer

Ex vivo upper mucosa

GeneSymbol

Description

1

− 0.1352253

10.349305

1.0953941

− 0.032011986

Lepr

Leptin receptor (Lepr), transcript variant 3, mRNA [NM_001122899]

2

− 1.7839279

9.163433

1.4320621

− 0.32384896

Bmp5

Bone morphogenetic protein 5 (Bmp5), mRNA [NM_007555]

0

8.42976

0.75516224

− 1.6026034

Bmp5

Bone morphogenetic protein 5 (Bmp5), mRNA [NM_007555]

3

− 5.845847

8.349145

0

− 1.0225489

Adamdec1

ADAM-like, decysin 1 (Adamdec1), mRNA [NM_021475]

4

− 0.036146164

7.296133

1.1356153

− 0.10349226

Pcdh12

Protocadherin 12 (Pcdh12), mRNA [NM_017378]

5

0.8064318

7.193366

0

− 1.9055712

Gli1

GLI-Kruppel family member GLI1 (Gli1), mRNA [NM_010296]

6

− 0.31227016

7.19128

0

− 1.1451445

Col15a1

Collagen, type XV, alpha 1 (Col15a1), mRNA [NM_009928]

7

− 0.25886297

6.7288184

1.0249934

− 0.65438557

Syne1

Spectrin repeat containing, nuclear envelope 1 (Syne1), transcript variant 5, mRNA [NM_001347732]

8

0.40697193

6.3136005

0

− 1.2896981

Lin7a

Lin-7 homolog A (C. elegans) (Lin7a), transcript variant 1, mRNA [NM_001039354]

9

4.5943704

6.2623343

0

− 2.2177994

Stxbp6

Syntaxin binding protein 6 (amisyn) (Stxbp6), mRNA [NM_144552]

10

0

6.2549396

1.8562856

− 1.847434

Grrp1

Glycine/arginine rich protein 1 (Grrp1), mRNA [NM_001099296]

11

1.4523826

5.9267335

0

− 1.1692836

Cadm3

Cell adhesion molecule 3 (Cadm3), mRNA [NM_053199]

12

0.21515155

5.9007926

0

− 2.616846

–

UI-M-EX0-byj-o-02-0-UI.r1 NIH_BMAP_EX0 cDNA clone IMAGE: 5,719,129 5′, mRNA sequence [CB248850]

13

0.17255163

5.841345

0

− 1.2357674

Agtr1b

Angiotensin II receptor, type 1b (Agtr1b), mRNA [NM_175086]

14

2.6327944

5.812486

0

− 1.8589058

Irf8

Interferon regulatory factor 8 [Source:MGI Symbol;Acc:MGI:96395] [ENSMUST00000162775]

15

− 2.760572

5.737793

1.2296844

− 0.068935394

Prdm6

PR domain containing 6 (Prdm6), mRNA [NM_001033281]

16

− 1.346303

5.5345974

0.86455584

− 1.5482342

Foxl1

Forkhead box L1 (Foxl1), mRNA [NM_008024]

17

− 1.9157376

5.5081615

1.5659752

− 0.92323875

Negr1

Neuronal growth regulator 1 (Negr1), transcript variant 1, mRNA [NM_001039094]

18

− 2.9770136

5.4516544

1.3360395

− 0.24065113

Bmp4

Bone morphogenetic protein 4 (Bmp4), transcript variant 1, mRNA [NM_007554]

19

− 3.0122108

5.441319

0.7840228

− 0.26249528

Bean1

Brain expressed, associated with Nedd4, 1 (Bean1), transcript variant 1, mRNA [NM_001141922]

20

− 0.8304815

5.440546

0.45523453

− 0.61619663

Ltbp4

Latent transforming growth factor beta binding protein 4 (Ltbp4), transcript variant 1, mRNA [NM_175641]

21

− 4.281811

5.415837

0.8549876

− 0.26267695

Tcf21

Transcription factor 21 (Tcf21), mRNA [NM_011545]

22

0.30940437

5.3867254

0

− 1.0120187

Cntn4

Contactin 4 (Cntn4), transcript variant 1, mRNA [NM_001109749]

23

− 0.0352149

5.2685137

0.8375006

− 0.8934727

Hcar1

Hydrocarboxylic acid receptor 1 (Hcar1), mRNA [NM_175520]

24

− 1.1227269

5.265641

0.48159218

− 1.4391389

Negr1

Neuronal growth regulator 1 (Negr1), transcript variant 2, mRNA [NM_177274]

25

0.41755295

5.2641068

0

− 1.005084

Tril

TLR4 interactor with leucine-rich repeats (Tril), mRNA [NM_025817]

26

− 0.65405416

5.2553177

0.27284336

− 1.1687546

Bend5

BEN domain containing 5 (Bend5), transcript variant 1, mRNA [NM_026279]

27

0.6547599

5.2338214

0

− 1.1381359

Ripply3

Ripply transcriptional repressor 3 (Ripply3), mRNA [NM_133229]

28

0

5.189296

1.0285192

− 0.7719319

Wnt2

Wingless-type MMTV integration site family, member 2 (Wnt2), mRNA [NM_023653]

29

0

5.1865263

0.31024742

− 1.5084703

Efcc1

EF hand and coiled-coil domain containing 1 [Source:MGI Symbol;Acc:MGI:3611451] [ENSMUST00000160743]

30

1.6226387

5.186082

0

− 1.0252223

Plac9a

Placenta specific 9a (Plac9a), mRNA [NM_207229]

  1. Results from in vivo- and ex vivo-expanded mesenchymal cells from the upper mucosa and crypt are shown. Top 30 genes of in vivo mesenchymal cells isolated from cryptal layers are shown.