Figure 2 | Scientific Reports

Figure 2

From: Development of a protein microarray-based diagnostic chip mimicking the skin prick test for allergy diagnosis

Figure 2

(A) ToF–SIMS ion distribution maps for the characteristic proline fragment (m/z 70 C4H8N+) on aldehyde-, epoxy- and NHS ester-coated surfaces. Each image was normalised to the corresponding total ion map. Brighter colour corresponds to higher normalised ion intensity. (B) Statistic Plots displaying normalised proline (C4H8N+) ion intensities of the three proteins (anti-human IgE, timothy grass pollen extract and κ-casein) printed on aldehyde-, epoxy- and NHS ester-coated surfaces. Data expressed are mean ± SD from quadruple determinations. Asterisks show results of Two-way ANOVA followed by Šídák’s multiple comparisons test; *p < 0.01, ****p < 0.0001.

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