Figure 4
From: Genome editing of CCR5 by CRISPR-Cas9 in Mauritian cynomolgus macaque embryos
Genotyping of CCR5-editing in whole MCM embryos. (A) Schematic of CRISPR-Cas9 targeting design. Representative gel electrophoresis images of the CCR5 amplicon in (B) whole embryos and (C) individual blastomeres of a single embryo. The wild-type (WT) product is 613 bp, whereas the 198 bp deletion results in a 415 bp product. An asterisk denote a homozygote. P1 primer 1, P2 primer 2, MT mutation, NC negative control, WT wild-type positive control. A 100 bp ladder was used.
