Table 1 Summary of in vitro development of cynomolgus macaque embryos.

From: Genome editing of CCR5 by CRISPR-Cas9 in Mauritian cynomolgus macaque embryos

 

Total from all experiments

Total from experiments with no embryo transfer

Oocytes

Cleaved

Cleavage rate (%)

Blastocysts

Oocytes

Cleaved

Cleavage rate (%)

Blastocysts

Blastocyst rate (%)

MCM

Control

44

32

72.7a

16

19

16

84.21

3

19.8

Cas9

65

32

49.2

0

40

13

32.5

0

0

CCR5 RNP

131

60

45.8a

3

78

15

19.2

1

6.7

CCM

Control

37

29

78.4b

0

22

7

31.8

0

0

Cas9

40

21

52.5

2

19

3

15.8

2

67

CCR5 RNP

85

32

37.6b

0

2

2

11.76

0

0

  1. The total number of oocytes fertilized by ICSI, cleaved embryos or blastocyst stage embryos are represented in the portion of the table without shading; since some embryos were removed for embryo transfer it is not possible to determine an accurate blastocyst rate. A pair-wise analysis using Fisher’s Exact Test was performed for each comparison within MCM or CCM experimental groups and a Bonferroni correction for multiple comparisons was applied to the p-value. A similar superscript denotes significance between the comparison: ap = 0.008, bp = 0.00018.
  2. MCM Mauritian cynomolgus macaque, CCM Chinese cynomolgus macaque, RNP CRISPR-Cas9 ribonucleoprotein complex.
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