Table 2 Timing of embryonic developmental events in cynomolgus macaque embryos cultured in vitro.

From: Genome editing of CCR5 by CRISPR-Cas9 in Mauritian cynomolgus macaque embryos

 

2-cell

h ± SD (n)

4-cell

h ± SD (n)

8-cell

h ± SD (n)

Morula

h ± SD (n)

Blastocyst

h ± SD (n)

Control

33.6 ± 12.1 (12)

47.7 ± 29.7 (9)

58.0 ± 20.5 (9)

106.0 ± 26.9 (6)

216.5 ± 47.4 (2)

Cas9

45.9 ± 19.6 (5)

50.9 ± 4.6 (8)

68.8 ± 35.9 (12)

110.0 ± 35.3 (6)

–

CCR5 RNP

56.6 ± 20.4 (15)

64.5 ± 22.0 (22)

81.7 ± 22.4 (19)

116.9 ± 28.9 (15)

177.0 (1)

  1. The time for each cleavage or developmental event is represented in hours, where the time of ICSI is time 0 h. The mean time ± SD is represented with the number of embryos analyzed in parenthesis. A two-way ANOVA resulted in no significant differences between embryo groups or developmental time points. The time to the blastocyst stage was not statistically evaluated given the limited sample number.
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