Table 4 CCR5 genotyping of individual blastomeres from CRISPR-Cas9 microinjected embryos.
From: Genome editing of CCR5 by CRISPR-Cas9 in Mauritian cynomolgus macaque embryos
Total cells | Number of cells: | Genotype | ||||
|---|---|---|---|---|---|---|
Homozygote | Heterozygote | Wild-type | No PCR signal | |||
Embryo1 | 8 | 0 | 6 | 1 | 1 | Heterozygote, mosaic |
Embryo2 | 8 | 0 | 4 | 0 | 4 | Heterozygote |
Embryo3 | 7 | 1 | 3 | 2 | 1 | Heterozygote, mosaic |
Embryo4 | 6 | 0 | 3 | 2 | 1 | Heterozygote, mosaic |
Embryo5 | 9 | 4 | 1 | 0 | 4 | Heterozygote, mosaic |
Embryo6 | 7 | 3 | 2 | 1 | 1 | Heterozygote, mosaic |
Embryo7 | 2 | 0 | 0 | 2 | 0 | Wild-type |
Embryo8 | 9 | 2 | 2 | 3 | 2 | Heterozygote, mosaic |
Embryo9 | 4 | 1 | 0 | 0 | 3 | Homozygote |
Embryo10 | 1 | 0 | 0 | 0 | 1 | Unknown |
Embryo11 | 4 | 1 | 0 | 0 | 3 | Homozygote |
Embryo12 | 4 | 2 | 0 | 0 | 2 | Homozygote |
Embryo13 | 5 | 0 | 0 | 3 | 2 | Wild-type |
Embryo14 | Multiple* | 1 | 0 | 0 | 0 | Homozygote |
Embryo15 | 13 | 4 | 3 | 1 | 5 | Heterozygote, mosaic |
Embryo16 | 14 | 3 | 3 | 2 | 6 | Heterozygote, mosaic |
Embryo17 | 16 | 0 | 0 | 13 | 3 | Wild-type |
Embryo18 | 12 | 5 | 1 | 2 | 5 | Heterozygote, mosaic |
Total | 129 | 27 | 28 | 32 | 43 | |
