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Figure 2

From: OSM-9 and OCR-2 TRPV channels are accessorial warm receptors in Caenorhabditis elegans temperature acclimatisation

Figure 2

Temperature acclimatisation assay. Animals were assayed using the 15 °C → 25 °C (0, 3, or 5 h) → 2 °C protocols. (a,b,c) TRPV mutants osm-9(ky10), ocr-2(ak47), osm-9(ky10) ocr-2(ak47) and osm-9(ky10) ocr-2(ak47); ocr-1(ak46) showed abnormally elevated survival rate (Number of assays ≥ 9, mean ± SEM). Comparisons were performed with Dunnett’s test for each condition: 15 °C → 25 °C (0, 3, 5 h) → 2 °C (**p < 0.01). (d,e) The abnormally elevated cold tolerance of osm-9 and ocr-2 mutants was partially rescued by expression of osm-9 and ocr-2 cDNA in ADL sensory neurons, respectively (number of assays ≥ 9, mean ± SEM). Statistical significance was assessed using ANOVA followed by a Bonferroni multi-comparison test (n.s. p ≥ 0.05, **p < 0.01, *p < 0.05).

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