Figure 3
Ca2+ imaging of ADL sensory neurons in TRPV mutants. Average thermal responses in ADL of each strain cultivated at 15 °C. Line graphs indicate YFP/CFP ratio changes under warming and cooling. (a) Data for wild-type in left and middle graphs are the same, given that the experiments were conducted simultaneously. Comparisons were performed with Dunnett’s test (n.s. p ≥ 0.05, **p < 0.01). The bar graph indicates the average ratio change from 230 to 235 s, a maximum point of ratio changes in wild-type (n ≥ 22, mean ± SEM). (b, c) Abnormal temperature responses of ADL in osm-9 and ocr-2 mutants were rescued by expression of osm-9 and ocr-2 cDNA in ADL, respectively. The bar graph indicates the average ratio change from 230 to 235 s (n ≥ 30, mean ± SEM). Statistical significance was assessed using ANOVA followed by a Bonferroni multi-comparison test (n.s. p ≥ 0.05, **p < 0.01, *p < 0.05).
