Figure 4
Ca2+ imaging of a gustatory neuron additively expressing OSM-9 and OCR-2. osm-9 cDNA or osm-9 cDNA with ocr-2 cDNA were expressed in a non-warmth-sensing gustatory neuron, ASER of a glr-3 mutant lacking a cold receptor GLR-3. Ca2+ imaging was performed using G-CaMP8. Line graphs indicate the G-CaMP8/tagRFP ratio change under warming. The bar graph indicates the average ratio change from 181 to 200 s, a temperature maximum point (n ≥ 27, mean ± SEM). Comparisons were performed with Dunnett’s test (n.s. p ≥ 0.05, **p < 0.01).
