Figure 4

Effects of scaffolds on cell proliferation in vitro. CCK-8 assays of rBMSCs with (A) BGC, (B) BGC-Mg, (C) Mg scaffold respectively at different culture time. (D) Fluorescene images of rBMSCs stained with calcein-AM (live cells, green fluorescence) and PI (dead cells, red fluorescence) with scaffolds at different culture time. (E) SEM micrographs of rBMSCs proliferated on BGC after 1 d of culture, and yellow arrowheads indicate the rBMSC cells on BGC. Cells in the control groups were cultured in normal medium.