Figure 1
From: A novel bioassay for quantification of surface Cannabinoid receptor 1 expression
Schematic overview of the engineered system and resulting protein used throughout this study. (A) The vector is designed to express GLuc with full-length CNR1 linked by a short sequence (linker) which was either the flexible domain (GLEG) or a FLAG tag (DYKDDDDK). The vector also contains the endogenous long 3′UTR of CNR1; (B) resulting protein containing GLuc (with signaling peptide, SP) fused via a linker to the N terminus of CB1 receptor. P promoter, SP signaling peptide, CNR1- cannabinoid receptor 1 gene, CB1- cannabinoid receptor 1 protein, Linker: either flexible domain (GLEG) or FLAG tag sequence (DYKDDDDK), 3′UTR untranslated region, ORI origin of replication, AmpR ampicillin resistance. (C) Schematic representation of the proposed CB1 quantification bioassay. CB1 fused to GLuc is measured by luciferase activity under the presence of coelenterazine.
