Figure 2 | Scientific Reports

Figure 2

From: Visualization and quantification of dynamic intercellular coupling in human embryonic stem cells using single cell sonoporation

Figure 2

Single cell loading of propidium iodide (PI) by sonoporation and cell–cell dye transfer via gap junctions (GJs) in hESCs. (A) Bright field image of hESCs before sonoporation with a cell-attached microbubble (arrow). (B) Bright field image of hESCs after sonoporation. (C) PI fluorescent image before sonoporation. (D) PI fluorescent image of hESCs after sonoporation at steady state, showing the uptake of PI in the sonoporated cell (arrow) and the surrounding cells after cell–cell dye transfer via GJs.

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