Figure 3

Effect of CNP on the levels of cAMP in bovine cumulus cells and oocytes. COCs derived from small follicles (SFs) and medium follicles (MFs) were cultured for 6 h in basal medium without or with 200 nM CNP. The levels of cAMP in bovine cumulus cells and oocytes were detected at the end of culture. (A) The levels of cAMP in bovine cumulus cells. (B) The levels of cAMP in bovine oocytes. Graphs show the mean ± SEM values of four independent experiments; The asterisks represent significant differences between the two groups with the same size follicle (P < 0.05).