Figure 4 | Scientific Reports

Figure 4

From: Effects of C-type natriuretic peptide on meiotic arrest and developmental competence of bovine oocyte derived from small and medium follicles

Figure 4

Inhibition of NPR2 abolishes the effect of CNP on the levels of cGMP/cAMP in bovine cumulus cells and oocytes. COCs derived from ovarian antral follicle were cultured in basal medium in the absence or presence of 200 nM CNP and/or 5 μM Gö6976, a competitive inhibitor of NPR2 (Basal medium, CNP, Gö6976, and CNP + Gö6976, respectively) for 6 h. The levels of cGMP/cAMP in bovine cumulus cells and oocytes were detected at the end of culture. (A) The levels of cGMP in bovine cumulus cells. (B) The levels of cGMP in bovine oocytes. (C) The levels of cAMP in bovine cumulus cells. (D) The levels of cAMP in bovine oocytes. Graphs show the mean ± SEM values of four independent experiments; Values indicated by different letters are significantly different (P < 0.05).

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