Figure 5

Silencing of DDR1 reduces HSCs secretion of chemotactic factors for both LSECs and C26 cells, and proliferative factors for C26 cells. (a) Western blot analysis of DDR1 in HSCs silenced (siDDR1) or not (siØ) for DDR1mRNA. (b) Histograms on computer-assisted semi-quantification of the Western Blot. LSECs or C26 cells were seeded in a collagen-coated upper well of a modified Boyden chamber to analyze their invasion rate in response to the chemotactic effect of SC secretomes located in the lower chamber. Control cells were allowed to migrate in response to basal media. (c,d) Chemotactic migration of LSECs (c) or C26 cells (d) in response to HSCs secretomes. (e) Proliferation rate of C26 cells in response to HSCs secretomes. Data are expressed as means ± SD. *P < 0.1, **/^##P < 0.01, ^###P < 0.001. Each experiment utilized cells from an independent isolation, and the western blots were processed in parallel. Full-length blots are presented in Supplementary Figure S8.