Figure 7
From: Silencing of sinusoidal DDR1 reduces murine liver metastasis by colon carcinoma
Immunohistochemical analysis of experimental hepatic metastasis by C26 cells in mice pretreated or not with DDR1 silencing RNA. (a) Immunofluorescence images of intratumoral expression of CD31/desmin/α-SMA in triple-labeled liver sections, and F4/80, Picrosirius Red, and in situ zymography in serial liver sections. (b) Representative images of DAPI and Ki67 staining in serial liver tissue sections. Liver tissue sections were obtained from the mouse analyzed in Fig. 6. T tumor, S sinusoids. Dotted white lines separate the tumor from the surrounding hepatic tissue. Scale bar 50 µm. (c) Histogram on computer-assisted semi-quantitation represents averaged values of 20 tumor foci per mice, in 24 mice with liver metastases. Data are expressed as means ± SD *P < 0.1, **P < 0.01, ***P < 0.001. The experiment was repeated twice. All images were processes under the same conditions.
