Figure 4
From: Genetic signatures of high-altitude adaptation and geographic distribution in Tibetan sheep
Comparison on sequence alignments and expression pattern between HAG1 and CSDE1. A: Secondary structure of HAG1 and differentiated amino acids between HAG1 and CSDE1. The location of 7 differentiated amino acids were depicted in symbol “*”, which are majorly on beta-strand and turn. Secondary structure of protein is predicted via Uniprot database from different PDB entries. B: Differentiated location of amino acids on representative 3D protein structure; C-D: Comparison on expression levels of CSDE1 and HAG1 in given 6 indigenous Tibetan sheep populations in this study. The 6 indigenous Tibetan sheep populations include Guide Black Fur sheep (GD), Qilian White Tibetan sheep (QL), Minxian Black Fur sheep (MX), Ganjia Tibetan sheep (GJ), Huoba Tibetan sheep (HB) and Awang Tibetan sheep (AW). Expression levels were calculated against GAPDH using mRNA from live and lung tissues, and four biological replicates were conducted.
