Figure 2
From: Inhibition of MUC1 exerts cell-cycle arrest and telomerase suppression in glioblastoma cells
MUC1 Knockdown inhibits the proliferation of glioblastoma cells. (A) GFP fluorescence images (magnification, × 100), RT-PCR and western blot (WB) analysis to confirm MUC1 depletion in U373 and T98G with shLuc or shMUC1 lentivirus infection. GADPH and ACTB were used as an internal control in RT-PCR and western blot, respectively. The blots were cropped and full length blots are presented in Supplementary Fig. S1. (B) Cell proliferation assay for 4 days and (C) Colony-formation assays with T98G and U373 cells transduced with shLuc or shMUC1 lentivirus. After 14 days, the cultured cells were fixed with methanol and stained simultaneously with staining solution (0.5% crystal violet in acetic acid/methanol 1:7) and colonies were counted manually. (B,C) were representative results from at least 3 independent experiments and were plotted with average of 3 independent experiments. Error bars, SEM. Student’s t-test. *P < 0.05, **P < 0.01, ***P < 0.001.
