Figure 4
From: Inhibition of MUC1 exerts cell-cycle arrest and telomerase suppression in glioblastoma cells
MUC1 knockdown is associated with the cell cycle and telomere maintenance pathway. (A) Gene set enrichment analysis (GSEA) of control and MUC1 knockdown glioblastoma (GBM) cell lines (T98G, U373, A172). TGF- β receptor signaling in epithelial mesenchymal transition (EMT) positively enriched in MUC1 knockdown cell whereas gene sets associated with cell cycle and telomere maintenance pathway are negatively enriched. “N” indicates gene counts in enriched gene set. The asterisks indicate FDR q-values (*< 0.25; **< 0.1; ***< 0.05; ****< 0.005; ***** < 0.0005). Pathways with p-value < 0.05 and NES score of > 2 or < -2 are shown. (B) Examples of GSEA charts revealing the role of MUC1 in GBM. Enrichment plot showing negative enrichment of cell cycle and telomere maintenance pathways and positive enrichment of TGF- β signaling in EMT pathway between control and MUC1 knockdown cell lines. (C) Enrichment map was drawn using Cytoscape with the GSEA result (C5 gene set from MSigDb), FDR cut off value 0.01, Edge cut off 0.5. Cell cycle pathways and telomere related pathways are interconnected together by common genes and all are negatively enriched in the MUC1 knockdown cell lines. (D) Heatmap comparison of cell cycle, telomere maintenance and EMT related gene lists in control vs MUC1 knockdown GBM cell lines.
